siRNA / miRNA gene silencing Human - U251 FAK

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

FAK siRNA (h)

Santa Cruz Biotechnology

Santa Cruz Biotechnology

Upstream tips	Protocol tips	Downstream tips
Seed 2 × 10^5	Add the siRNA duplex solution (Solution A) directly to the dilute Transfection Reagent (Solution B) using a pipette. Mix gently by pipetting the solution up and down and incubate the mixture 15-45 minutes at room temperature. Wash the cells once with 2 ml of siRNA Transfection Medium. Add siRNA Transfection Reagent mixture (Solution A + Solution B) to cells and incubate the cells 5-7 hours at 37° C in a CO2 incubator and replace medium. Incubate the cells for an additional 18-24 hours.

Upstream tips
Seed 2 × 10^5
Protocol tips
Add the siRNA duplex solution (Solution A) directly to the dilute Transfection Reagent (Solution B) using a pipette. Mix gently by pipetting the solution up and down and incubate the mixture 15-45 minutes at room temperature. Wash the cells once with 2 ml of siRNA Transfection Medium. Add siRNA Transfection Reagent mixture (Solution A + Solution B) to cells and incubate the cells 5-7 hours at 37° C in a CO2 incubator and replace medium. Incubate the cells for an additional 18-24 hours.

Manufacturer protocol Publication protocol 1 Relevant paper

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