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|Seed 2 × 10^5|
|Add the siRNA duplex solution (Solution A) directly to the dilute Transfection Reagent (Solution B) using a pipette. Mix gently by pipetting the solution up and down and incubate the mixture 15-45 minutes at room temperature.
Wash the cells once with 2 ml of siRNA Transfection Medium.
Add siRNA Transfection Reagent mixture (Solution A + Solution B) to cells and incubate the cells 5-7 hours at 37° C in a CO2 incubator and replace medium.
Incubate the cells for an additional 18-24 hours.