siRNA / miRNA gene silencing Human - U87MG HES6

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Found 1 matching solution for this experiment

Hs_HES6_1 FlexiTube siRNA. + FuGENE® 6 Transfection Reagent

Protocol tips
siRNA-Hs_HES6_1: TTGGAGTTAGTTACCCTTGAA

Final concentration of 15 nM each, or as a pool of three siRNAs (denoted as siHES6) 10 nM each.

Incubate the FuGENE 6 Transfection Reagent/medium mixture for 5 minutes at room temperature 

Incubate transfection mixture for 15 min at room temperature and add to cells and shake for 10-30 seconds. 

Incubate cells for 24-48 h. 
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