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Final concentration of 15 nM each, or as a pool of three siRNAs (denoted as siHES6) 10 nM each.
Incubate the FuGENE 6 Transfection Reagent/medium mixture for 5 minutes at room temperature
Incubate transfection mixture for 15 min at room temperature and add to cells and shake for 10-30 seconds.
Incubate cells for 24-48 h.