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Found 1 matching solution for this experiment
Hs_PLK1_7 FlexiTube siRNA + FuGENE® 6 Transfection Reagent
| Upstream tips |
Protocol tips |
Downstream tips |
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siRNA-Hs_PLK1_7: CGCGGGCAAGATTGTGCCTAA
Final concentration of 15 nM each, or as a pool of three siRNAs (denoted as siHES6) 10 nM each.
Incubate the FuGENE 6 Transfection Reagent/medium mixture for 5 minutes at room temperature
Incubate transfection mixture for 15 min at room temperature and add to cells and shake for 10-30 seconds.
Incubate cells for 24-48 h. |
|
| Protocol tips |
siRNA-Hs_PLK1_7: CGCGGGCAAGATTGTGCCTAA
Final concentration of 15 nM each, or as a pool of three siRNAs (denoted as siHES6) 10 nM each.
Incubate the FuGENE 6 Transfection Reagent/medium mixture for 5 minutes at room temperature
Incubate transfection mixture for 15 min at room temperature and add to cells and shake for 10-30 seconds.
Incubate cells for 24-48 h. |
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