siRNA / miRNA gene silencing Human - U937 HDAC5

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

Hs_HDAC5_4 FlexiTube siRNA

Qiagen

Upstream tips	Protocol tips	Downstream tips
	siRNA sequence: Sense CGGGUUUGAUGCUGUUGAATT AntiSense-UUCAACAGCAUCAAACCCGGC siRNA concentartion-120 pmol It is recommended to use HiPerFect Transfection Reagent for low-throughput siRNA transfection and HiPerFect HTS Reagent for high-throughput siRNA transfection.

Protocol tips
siRNA sequence: Sense CGGGUUUGAUGCUGUUGAATT AntiSense-UUCAACAGCAUCAAACCCGGC siRNA concentartion-120 pmol It is recommended to use HiPerFect Transfection Reagent for low-throughput siRNA transfection and HiPerFect HTS Reagent for high-throughput siRNA transfection.

Publication protocol 1 Relevant paper

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