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|Seed 0.5 × 10^5 cells per well in a 12-well plate.|
|siRNA TIMP-2 -100 pM
TIMP-2 siRNA #1 (5′-GAAGGAGUAUCUAAUUGCATT-3′), TIMP-2 siRNA #2 (5′-UCCACAGACUUCAGCGAAU-3′),
Mix diluted RNA and diluted siPORT™ NeoFX™ Transfection Agent; incubate 10 min at room temp,
Incubate the transfected cells at 37°C for 48h.
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