siRNA / miRNA gene silencing Mouse - BV2 TIMP-2

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 1 matching solution for this experiment

Timp2 siRNA + siPORT™ NeoFX™ Transfection Agent

Timp2 siRNA

Thermo Fisher Scientific

Upstream tips
Seed 0.5 × 10^5 cells per well in a 12-well plate.
Protocol tips
siRNA TIMP-2 -100 pM


Mix diluted RNA and diluted siPORT™ NeoFX™ Transfection Agent; incubate 10 min at room temp,

Incubate the transfected cells at 37°C for 48h.
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms