siRNA / miRNA gene silencing Mouse - Embryonic stem cells CXCR4

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

Cxcr4 siRNA

Thermo Fisher Scientific

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	Final concentartion of siRNA- 100 pmol per plate. Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Treat cells with Sdf-1 (10 ng/μl) after 24 h. Incubate cells for another 48h at 37°C and quantify.

Protocol tips
Final concentartion of siRNA- 100 pmol per plate. Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Treat cells with Sdf-1 (10 ng/μl) after 24 h. Incubate cells for another 48h at 37°C and quantify.

Manufacturer protocol Publication protocol 1 Relevant paper

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