siRNA / miRNA gene silencing Mouse - Neuro 2a TLR3

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

Rn_Tlr3_1 FlexiTube siRNA

Qiagen

Qiagen

Upstream tips	Protocol tips	Downstream tips
Seed 2×10^4 cells	siRNA concentration-10 nM Incubate HiPerFect Transfection Reagent to diluted siRNA for 10–15 min at RT. 24 h post transfection treat cells with infected with 0.1 MOI of JEV or 1 μg/μLof TLR3 ligand poly I:C(positive control) and incubate for 1h and replace medium. Analyse cells after 18h

Upstream tips
Seed 2×10^4 cells
Protocol tips
siRNA concentration-10 nM Incubate HiPerFect Transfection Reagent to diluted siRNA for 10–15 min at RT. 24 h post transfection treat cells with infected with 0.1 MOI of JEV or 1 μg/μLof TLR3 ligand poly I:C(positive control) and incubate for 1h and replace medium. Analyse cells after 18h

Manufacturer protocol Publication protocol 1 Relevant paper

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