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|Seed 2×10^4 cells|
|siRNA concentration-10 nM
Incubate HiPerFect Transfection Reagent to diluted siRNA for 10–15 min at RT.
24 h post transfection treat cells with infected with 0.1 MOI of JEV or 1 μg/μLof TLR3 ligand poly I:C(positive control) and incubate for 1h and replace medium.
Analyse cells after 18h