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|Add Solution A to Solution B and incubate for 15-45 minutes at room temperature.
Add this mixture to cells and incubate for 5-7 hours at 37° C in a CO2 incubator.
Add 1 ml of normal growth medium containing 2 times the normal serum and antibiotics concentration (2x normal growth medium) without removing the transfection mixture.
Incubate the cells for an additional 18-24 hours.