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|Seed 2×10^5 cells per well in a six-well plate|
|Add solution -A and solution-B and incubate the mixture 15-45 minutes at room temperature.
Add the mixture to cells and incubate the cells 5-7 hours at 37° C in a CO2 incubator and later add normal growth medium and incubate for an additional 18-24 hours.
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