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Found 1 matching solution for this experiment
Ccr2 siRNA + Lipofectamine® 2000 Transfection Reagent
| Upstream tips |
Protocol tips |
Downstream tips |
|
Dilute Lipofectamine 2000 in DMEM/F12.
Combine the above mixture with 0.01 to 0.20 µg siRNA
Incubate for 15min at RT.
Incubate cells for 1 day at 37°C |
Analyze transfected cells with western blot analysis. |
| Protocol tips |
Dilute Lipofectamine 2000 in DMEM/F12.
Combine the above mixture with 0.01 to 0.20 µg siRNA
Incubate for 15min at RT.
Incubate cells for 1 day at 37°C |
| Downstream tips |
| Analyze transfected cells with western blot analysis. |
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