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Found 1 matching solution for this experiment
Slc1a2
Thermo Fisher Scientific
| Upstream tips |
Protocol tips |
Downstream tips |
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GLT-1 (#1: UAACUUCAUGACAAUCUCGTT, #2:UCGUGGACAUGUAAUAUACAA)
Seed 3 x 10^5 cells/well in a 12 well plate.
Transfect with 1 μg siRNA in a media containing 5mM glutamate and incubate at 37°C with 5% CO2 overnight.
For 7 days knockdown experiment, transfect cells on day 0 and day 3. |
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| Protocol tips |
GLT-1 (#1: UAACUUCAUGACAAUCUCGTT, #2:UCGUGGACAUGUAAUAUACAA)
Seed 3 x 10^5 cells/well in a 12 well plate.
Transfect with 1 μg siRNA in a media containing 5mM glutamate and incubate at 37°C with 5% CO2 overnight.
For 7 days knockdown experiment, transfect cells on day 0 and day 3. |
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