No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 1 matching solution for this experiment
Thermo Fisher Scientific
|GLT-1 (#1: UAACUUCAUGACAAUCUCGTT, #2:UCGUGGACAUGUAAUAUACAA)
Seed 3 x 10^5 cells/well in a 12 well plate.
Transfect with 1 μg siRNA in a media containing 5mM glutamate and incubate at 37°C with 5% CO2 overnight.
For 7 days knockdown experiment, transfect cells on day 0 and day 3.
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product