siRNA / miRNA gene silencing Rat - Glial cells GLT-1

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Found 1 matching solution for this experiment

Slc1a2 + I-FECT

Slc1a2

Thermo Fisher Scientific

I-FECT

Neuromics

Protocol tips
GLT-1 (#1: UAACUUCAUGACAAUCUCGTT, #2:UCGUGGACAUGUAAUAUACAA)

Seed 3 x 10^5 cells/well in a 12 well plate.

Transfect with 1 μg siRNA in a media containing 5mM glutamate and incubate at 37°C with 5% CO2 overnight.

For 7 days knockdown experiment, transfect cells on day 0 and day 3.
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