siRNA / RNAi /miRNA transfection Mouse - Glomerular Mesangial cells polymer / lipid

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

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Found 1 matching solution for this experiment

TaqMan® MicroRNA Reverse Transcription Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
Use 1 to 10 ng of total RNA per 15- µL RT reaction	Treat RT mixture for 30 minutes at 16C , 30 minutes at 42C and to inactivate treat the tube at 85C for 5 minutes

Upstream tips
Use 1 to 10 ng of total RNA per 15- µL RT reaction
Protocol tips
Treat RT mixture for 30 minutes at 16C , 30 minutes at 42C and to inactivate treat the tube at 85C for 5 minutes

Manufacturer protocol Publication protocol 1 Relevant paper

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