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Found 2 matching solutions for this experiment
|Incubate the mixed reagents for 30 min at room temperature prior to use.
Incubate cells with siRNA transfection reagent mixture (solution A + solution B) for 6 h at 37°C in a CO2 incubator and remove to replace 1X normal growth medium for an additional 24 h.
|Dilute siRNA in culture medium without serum.
Incubate the samples for 5–10 min at room temperature
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