Site Directed Mutagenesis (SDM) Human - Point mutation 786-O REGγ

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

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Found 1 matching solution for this experiment

Site-Directed Mutagenesis Kit

SBS Genetech

Upstream tips	Protocol tips	Downstream tips
In case of using much amount of plasmid DNA, sometimes Mutazyme™ Enzyme couldn’t reaction with sample. So we suggest you to follow the procedure correctly for good mutation efficiency.

Upstream tips
In case of using much amount of plasmid DNA, sometimes Mutazyme™ Enzyme couldn’t reaction with sample. So we suggest you to follow the procedure correctly for good mutation efficiency.

Manufacturer protocol Publication protocol 1 Relevant paper

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