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Found 3 matching solutions for this experiment
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RPMI-1640 (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) plus antibiotics (100 U/mL penicillin and 100 mg/mL streptomycin) |
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RPMI-1640 (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) plus antibiotics (100 U/mL penicillin and 100 mg/mL streptomycin) |
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DMEM:F12 (1:1) medium (Invitrogen) supplemented with 2 mM l-glutamine and 20% heat-inactivated foetal bovine serum |
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DMEM:F12 (1:1) medium (Invitrogen) supplemented with 2 mM l-glutamine and 20% heat-inactivated foetal bovine serum |
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IMDM (Biochrome, Merck, Berlin, Germany) supplemented with growth factors: 50 ng/ml stem cell factor (SCF), 50 ng/ml TPO, 1 ng/ml IL-3 and 20 ng/ml IL-6. 4 days later the growth factor mix was changed to 50 ng/ml TPO and 10 ng/ml IL-1β as described previously by Heideveld et al. (all from PeproTech, Pittsburgh, USA) |
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IMDM (Biochrome, Merck, Berlin, Germany) supplemented with growth factors: 50 ng/ml stem cell factor (SCF), 50 ng/ml TPO, 1 ng/ml IL-3 and 20 ng/ml IL-6. 4 days later the growth factor mix was changed to 50 ng/ml TPO and 10 ng/ml IL-1β as described previously by Heideveld et al. (all from PeproTech, Pittsburgh, USA) |
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