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Found 2 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
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Advanced DMEM/F12 supplemented with 10 × 10−3 m HEPES, 1x Glutamax, 1% Penicillin/Streptomycin, 1x B27, 1.25 × 10−3 m N ‐acetylcysteine, 50% Wnt3a CM (produced in‐house), 20% R‐spondin‐1 CM, 10% noggin CM, 10 × 10−3 m nicotinamide (Sigma), 50 ng mL−1 human EGF, 10 × 10−9 m Gastrin (Tocris Bioscience), 0.5 × 10−6 m A83‐01 (Tocris Bioscience) and 10 × 10−6 m SB202190 (Sigma) |
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Protocol tips |
Advanced DMEM/F12 supplemented with 10 × 10−3 m HEPES, 1x Glutamax, 1% Penicillin/Streptomycin, 1x B27, 1.25 × 10−3 m N ‐acetylcysteine, 50% Wnt3a CM (produced in‐house), 20% R‐spondin‐1 CM, 10% noggin CM, 10 × 10−3 m nicotinamide (Sigma), 50 ng mL−1 human EGF, 10 × 10−9 m Gastrin (Tocris Bioscience), 0.5 × 10−6 m A83‐01 (Tocris Bioscience) and 10 × 10−6 m SB202190 (Sigma) |
Upstream tips |
Protocol tips |
Downstream tips |
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DMEM/F12 medium (Invitrogen) containing 1 × B27 (Invitrogen), 500 ng/ml R-Spondin 1 (R&D Systems), 100 ng/ml EGF (R&D Systems), and 100 ng/ml Noggin (R&D Systems) |
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Protocol tips |
DMEM/F12 medium (Invitrogen) containing 1 × B27 (Invitrogen), 500 ng/ml R-Spondin 1 (R&D Systems), 100 ng/ml EGF (R&D Systems), and 100 ng/ml Noggin (R&D Systems) |
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