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Found 3 matching solutions for this experiment
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DMEM/Ham'sF-12 (Biochrom, Berlin, Germany) supplemented with stabile glutamine (Sigma-Aldrich, Munich, Germany), 10% FBS (PAN Biotech, Aidenbach, Germany), 1% penicillin/streptomycin (Sigma-Aldrich), 1% MEM (Biochrom, Berlin, Germany), and 1% L-ascorbic acid-2-phosphate solution (Sigma-Aldrich) |
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DMEM/Ham'sF-12 (Biochrom, Berlin, Germany) supplemented with stabile glutamine (Sigma-Aldrich, Munich, Germany), 10% FBS (PAN Biotech, Aidenbach, Germany), 1% penicillin/streptomycin (Sigma-Aldrich), 1% MEM (Biochrom, Berlin, Germany), and 1% L-ascorbic acid-2-phosphate solution (Sigma-Aldrich) |
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LG-DMEM containing with 10% FBSÂ |
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LG-DMEM containing with 10% FBSÂ |
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HG-DMEM supplemented with 10% FBS (Sigma-Aldrich, St. Louis, MO, USA), 50 U/mL penicillin, 50 mg/mL streptomycin, 2 mM L-glutamine (all from Life Technologies), and maintained in incubator at 37 °C in a humidified atmosphere with 5% CO2. Once they reached 80â90% of confluence, the cells were detached with 0.05% trypsin/0.2% EDTA (Sigma-Aldrich) |
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HG-DMEM supplemented with 10% FBS (Sigma-Aldrich, St. Louis, MO, USA), 50 U/mL penicillin, 50 mg/mL streptomycin, 2 mM L-glutamine (all from Life Technologies), and maintained in incubator at 37 °C in a humidified atmosphere with 5% CO2. Once they reached 80â90% of confluence, the cells were detached with 0.05% trypsin/0.2% EDTA (Sigma-Aldrich) |
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