No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 3 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
plated onto 0.1% gelatin-coated culture dishes (5 × 105 cells per 9.6 cm2 for neonate and 5 × 105 cells per 9.6 cm2 for adult mice). |
StemPro-34 medium, N2-supplement, D+ glucose, bovine serum albumin, L-glutamine, β-mercaptoethanol, penicillin/streptomycin, MEM vitamins, NEAA, estradiol, progesterone, EGF, FGF, GDNF, LIF, ES-FBS, ascorbic acid, pyruvic acid, and DL-lactic acid |
|
Upstream tips |
plated onto 0.1% gelatin-coated culture dishes (5 × 105 cells per 9.6 cm2 for neonate and 5 × 105 cells per 9.6 cm2 for adult mice). |
Protocol tips |
StemPro-34 medium, N2-supplement, D+ glucose, bovine serum albumin, L-glutamine, β-mercaptoethanol, penicillin/streptomycin, MEM vitamins, NEAA, estradiol, progesterone, EGF, FGF, GDNF, LIF, ES-FBS, ascorbic acid, pyruvic acid, and DL-lactic acid |
Upstream tips |
Protocol tips |
Downstream tips |
|
Dulbecco's Modified Eagle's Medium/Nutrient Mixture F12 (DMEM/F12, Invitrogen, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS, Hyclone, USA), 2 mM L-glutamine (Invitrogen), and 100 unit/ml penicillin and streptomycin (Invitrogen) |
|
Protocol tips |
Dulbecco's Modified Eagle's Medium/Nutrient Mixture F12 (DMEM/F12, Invitrogen, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS, Hyclone, USA), 2 mM L-glutamine (Invitrogen), and 100 unit/ml penicillin and streptomycin (Invitrogen) |
Upstream tips |
Protocol tips |
Downstream tips |
|
α-MEM medium, supplemented with 10% FBS (Hyclone, Logan, UT, USA), 0.1 mM 2-mercaptoethanol (Invitrogen), 4 mM glutamine (Invitrogen), 1% non-essential amino acids (Invitrogen), 10 ng/ml GDNF (Peprotech, Rocky Hill, NJ, USA), 20 ng/ml EGF (Millipore, Temecula, CA, USA), 20 ng/ml bFGF (Millipore) and 1000 U/ml LIF (Millipore) |
|
Protocol tips |
α-MEM medium, supplemented with 10% FBS (Hyclone, Logan, UT, USA), 0.1 mM 2-mercaptoethanol (Invitrogen), 4 mM glutamine (Invitrogen), 1% non-essential amino acids (Invitrogen), 10 ng/ml GDNF (Peprotech, Rocky Hill, NJ, USA), 20 ng/ml EGF (Millipore, Temecula, CA, USA), 20 ng/ml bFGF (Millipore) and 1000 U/ml LIF (Millipore) |
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!