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Found 3 matching solutions for this experiment
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mixture of mouse TS medium (MEF)-conditioned with TS basal medium at a ratio of 7:3) supplemented with 25 ng/ml Fgf4 and 1 μg/ml of heparin |
TS basal medium RPMI (Roswell Park Memorial Institute) 1640 medium supplemented with 20% FBS, 100 μM β-mercaptoethanol, 2 mM L-glutamine, 1 mM sodium pyruvate, 50 U/ml penicillin/streptomycin |
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mixture of mouse TS medium (MEF)-conditioned with TS basal medium at a ratio of 7:3) supplemented with 25 ng/ml Fgf4 and 1 μg/ml of heparin |
Protocol tips |
TS basal medium RPMI (Roswell Park Memorial Institute) 1640 medium supplemented with 20% FBS, 100 μM β-mercaptoethanol, 2 mM L-glutamine, 1 mM sodium pyruvate, 50 U/ml penicillin/streptomycin |
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DMEM/F12 supplemented with 0.1 mM 2-mercaptoethanol, 0.2% FBS, 0.5% Penicillin-Streptomycin, 0.3% BSA, 1% ITS-X supplement, 1.5 μg/ml L-ascorbic acid, 50 ng/ml EGF, 2 μM CHIR99021, 0.5 μM A83-01, 1 μM SB431542, 0.8 mM VPA and 5 μM Y27632 |
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DMEM/F12 supplemented with 0.1 mM 2-mercaptoethanol, 0.2% FBS, 0.5% Penicillin-Streptomycin, 0.3% BSA, 1% ITS-X supplement, 1.5 μg/ml L-ascorbic acid, 50 ng/ml EGF, 2 μM CHIR99021, 0.5 μM A83-01, 1 μM SB431542, 0.8 mM VPA and 5 μM Y27632 |
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70% (v/v) mouse embryonic fibroblast-conditioned medium (MEF-CM) and 30% TS medium (RPMI 1640 supplemented with 20% FBS (Invitrogen), 0.5% penicillin-streptomycin (Thermo Fisher Scientific), 1% GlutaMAX (Thermo Fisher Scientific), 1% sodium pyruvate (Thermo Fisher Scientific) and 0.1 mM 2-mercaptoethanol (Thermo Fisher Scientific) that was supplemented with 20 μM Y-27632 (MCE), 37.5 ng/ml FGF4 (Sigma-Aldrich), and 1.5 μg/ml Heparin (Sigma-Aldrich) |
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70% (v/v) mouse embryonic fibroblast-conditioned medium (MEF-CM) and 30% TS medium (RPMI 1640 supplemented with 20% FBS (Invitrogen), 0.5% penicillin-streptomycin (Thermo Fisher Scientific), 1% GlutaMAX (Thermo Fisher Scientific), 1% sodium pyruvate (Thermo Fisher Scientific) and 0.1 mM 2-mercaptoethanol (Thermo Fisher Scientific) that was supplemented with 20 μM Y-27632 (MCE), 37.5 ng/ml FGF4 (Sigma-Aldrich), and 1.5 μg/ml Heparin (Sigma-Aldrich) |
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