No discussions found
Found 2 matching solutions for this experiment
|-The seeding density of cells should be optimized for the application and the cell line.|
|-Do not prepare the Poly-D-Lysine (PDL) solution with borate buffer. Increased cell clumping is observed when the PDL solution is prepared in borate buffer, and then cells are cultured in medium supplemented with NeuroCult™ SM1.
- Ensure that the coverslips are completely submerged in the PDL solution, as they tend to float. If this happens, use a sterile plastic pipette tip to push the coverslip to the bottom of the well.
|-If not used immediately, aliquot PDL stock solution into polypropylene vials and store at 2 - 8°C for up to 1 month.|