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Found 3 matching solutions for this experiment
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Cells were stimulated with specific differentiation media Stem Pro differentiation kits (Life technologies, USA) following manufactures protocol. |
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Cells were stimulated with specific differentiation media Stem Pro differentiation kits (Life technologies, USA) following manufactures protocol. |
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DMEM plus l-ascorbic acid-2-phosphate, pyruvic acid sodium salt (Sigma Aldrich), l-proline (Sigma Aldrich), ITS+ culture supplement (BD Bioscience, Erembodegem, Belgium) and TGF-β3 (R&D Systems, Abingdon, UK). |
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Protocol tips |
DMEM plus l-ascorbic acid-2-phosphate, pyruvic acid sodium salt (Sigma Aldrich), l-proline (Sigma Aldrich), ITS+ culture supplement (BD Bioscience, Erembodegem, Belgium) and TGF-β3 (R&D Systems, Abingdon, UK). |
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DMEM supplemented with 40 μg/mL proline, 50 µg/mL ITS-premix, 50 µg/mL AsAP, 100 µg /mL Sodium Pyruvate, 10 ng/mL TGFβ3, 10−7 M dexamethasone, 100 U penicillin/mL, and 100 μg/mL streptomycin |
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Protocol tips |
DMEM supplemented with 40 μg/mL proline, 50 µg/mL ITS-premix, 50 µg/mL AsAP, 100 µg /mL Sodium Pyruvate, 10 ng/mL TGFβ3, 10−7 M dexamethasone, 100 U penicillin/mL, and 100 μg/mL streptomycin |
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