No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 2 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
|
DMEM)-mega-cell, supplemented with 10% FBS, 2 mM glutamine, 100 U/ml penicillin-streptomycin amphotericin, 1% non-essential amino acids (Lonza, Switzerland), 0.1 mM β-mercaptoethanol (Serva, Germany), 5 ng/ml basic fibroblast growth factor (Gibco, USA) and 10 µM 5-Aza-2'-deoxycytidine (5-Aza) (Abcam, UK). |
|
Protocol tips |
DMEM)-mega-cell, supplemented with 10% FBS, 2 mM glutamine, 100 U/ml penicillin-streptomycin amphotericin, 1% non-essential amino acids (Lonza, Switzerland), 0.1 mM β-mercaptoethanol (Serva, Germany), 5 ng/ml basic fibroblast growth factor (Gibco, USA) and 10 µM 5-Aza-2'-deoxycytidine (5-Aza) (Abcam, UK). |
Upstream tips |
Protocol tips |
Downstream tips |
seeded on a Matrigel-coated dish at a concentration of 105 cells/cm2 |
DMEM supplemented with 2% horse serum |
|
Upstream tips |
seeded on a Matrigel-coated dish at a concentration of 105 cells/cm2 |
Protocol tips |
DMEM supplemented with 2% horse serum |
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!