Found 1 discussion for this experiment
2 years ago
2 years ago by Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
Found 6 matching solutions for this experiment
|Precoating slides with poly-L-lysine may enhance cell adherence.
Do not allow the samples to dry out.
All other FragELTM kit components, with the exception of the Mounting Media, should be kept on ice during usage then promptly returned to -20C
|Dilute 5X TdT Equilibration Buffer 1:5 with water.
Place slides in a humidified chamber and incubate at 37C for 1 - 1.5 hours after ading TdT Labeling Reaction Mixture
|Fix cell samples with Fixation solution for 1 h.
Incubate in Permeabilisation solution (0.1% Triton X-100 in PBS) for 2 min on ice.
Label and incubate for 60 min at +37°C in a humidified atmosphere in the dark
|If low labelling is found, reduce fixation time or try 2% buffered paraformaldehyde|