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1 year ago
1 year ago by Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
Found 6 matching solutions for this experiment
|Add Tunel mixture and incubate for 60 min at +37°C in a humidified atmosphere in the
|If no specific labelling, reduce concentration of TdT by diluting it 1:2 up to 1:10 with TUNEL Dilution Buffer.
If high background, reduce concentration of labeling mix
to 50% by diluting with TUNEL Dilution Buffer
|Fix cell samples with Fixation solution for 1 h.
Incubate in Permeabilisation solution (0.1% Triton X-100 in PBS) for 2 min on ice.
Label and incubate for 60 min at +37°C in a humidified atmosphere in the dark
|If low labelling is found, reduce fixation time or try 2% buffered paraformaldehyde|