Wound healing assay cell type - human A549

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

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2 years ago

2 years ago by Lauren Dobson United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

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Protocol tips
- NHBE ABI cultures were fixed in 10% neutral buffered formalin 0, 24 h, 48 h, 72 h, and 96 h. After wounding cells are stained with eosin and hematoxylin.
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