Wound healing assay cell type - human Caco-2

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

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4 years ago

4 years ago by Lauren Dobson United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

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Protocol tips
- After washing at step -7 different stimuli were added (Trx-lps, from S. ratti and T. suis, in concentrations of 3 ng, 30 ng, 300 ng, 1 μg, 10 μg, and 25 μg per 500 μL). As a positive control EGF; 0.5 ng, 5 ng, 10 ng, 15 ng, and 25 ng is added and as a negative control LPS were added.
Protocol tips
- Cells are washed after 24h incubation with pre-warmed, serum- and phenol-red-free DMEM (EGF and OPN were dissolved in DMEM+1% FBS v/v+1% v/v penicillin / streptomycin). After another 24h, wells are washed in DMEM without phenol red and stained with 2 µM SYTO 24® DNA stain and 3 µM propidium iodide for 30 min at 37 °C
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