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2 years ago
2 years ago by Lauren Dobson
I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?
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|- After washing at step -7 different stimuli were added (Trx-lps, from S. ratti and T. suis, in concentrations of 3 ng, 30 ng, 300 ng, 1 μg, 10 μg, and 25 μg per 500 μL). As a positive control EGF; 0.5 ng, 5 ng, 10 ng, 15 ng, and 25 ng is added and as a negative control LPS were added.|
|- Cells are washed after 24h incubation with pre-warmed, serum- and phenol-red-free DMEM (EGF and OPN were dissolved in DMEM+1% FBS v/v+1% v/v penicillin / streptomycin). After another 24h, wells are washed in DMEM without phenol red and stained with 2 µM SYTO 24® DNA stain and 3 µM propidium iodide for 30 min at 37 °C