Wound healing assay cell type - mouse MS1

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

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2 years ago

2 years ago by Lauren Dobson United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

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Found 1 matching solution for this experiment

Cell Comb™ Scratch Assay

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- Scratched monolayer is washed with Dulbecco’s phosphate-buffered saline to remove any detached cells.	- Post-wounding images are captured every 6h and between imaging sessions cells are returned to 37 °C, 5% CO2

Protocol tips
- Scratched monolayer is washed with Dulbecco’s phosphate-buffered saline to remove any detached cells.
Downstream tips
- Post-wounding images are captured every 6h and between imaging sessions cells are returned to 37 °C, 5% CO2

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