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Found 2 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
- Cells were pretreated with SiNPs at different concentrations (0, 25, 50, 100 μg/ml) for 24 h and 48 h. |
- Hydrogen peroxide 20 μM concentration was used for the assay.
- After adding DCFH, plate was incubated for 30min. |
|
Upstream tips |
- Cells were pretreated with SiNPs at different concentrations (0, 25, 50, 100 μg/ml) for 24 h and 48 h. |
Protocol tips |
- Hydrogen peroxide 20 μM concentration was used for the assay.
- After adding DCFH, plate was incubated for 30min. |
Upstream tips |
Protocol tips |
Downstream tips |
|
- Cells were treated with 25 μM DCFDA in essential medium with 10% FBS in 37°C incubator for 45 min.
- Before analysing cells, they are treated with hyperosmotic media by adding NaCl for different time periods (30–180 min). |
|
Protocol tips |
- Cells were treated with 25 μM DCFDA in essential medium with 10% FBS in 37°C incubator for 45 min.
- Before analysing cells, they are treated with hyperosmotic media by adding NaCl for different time periods (30–180 min). |
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