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Found 1 matching solution for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
Pre-warm the supplemented Nucleofector Solution
pre-incubate plates filled with 1.0 ml of culture medium containing serum and supplements in a humidified 37°C/5% CO2 incubator |
seed 1x10^6 – 5x10^6 cells in the wells containing above medium and centrifuge at 90xg for 10 min.
Resuspend pellet and add Nucleofector Solution to a final concentration of 1x106 – 5x106
cells/100 μl
Mix 100 μl of cell suspension with siRNA |
|
| Upstream tips |
Pre-warm the supplemented Nucleofector Solution
pre-incubate plates filled with 1.0 ml of culture medium containing serum and supplements in a humidified 37°C/5% CO2 incubator |
| Protocol tips |
seed 1x10^6 – 5x10^6 cells in the wells containing above medium and centrifuge at 90xg for 10 min.
Resuspend pellet and add Nucleofector Solution to a final concentration of 1x106 – 5x106
cells/100 μl
Mix 100 μl of cell suspension with siRNA |
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