crispr-mouse-activation-3t3-l1-c-ebp

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using hCas9 to perform CRISPR Mouse - Deletion 3T3-L1 ATP7A

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Deletion 3T3-L1 TEAD

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Deletion 3T3-L1 Epac1

Get tips on using pX333-sgNF1-sgTrp53-Cas9 to perform CRISPR Mouse - Deletion 3T3-L1 Trp53

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Deletion 3T3-L1 MmP13

Get tips on using pSPCas9(BB)-2A-GFP plasmids to perform CRISPR Mouse - Deletion 3T3-L1 Cep350

Get tips on using pSPCas9(BB)-2A-GFP plasmids to perform CRISPR Mouse - Deletion 3T3-L1 Usp2

Get tips on using pSpCas9(BB)-2A-Puro (PX459) V2.0 to perform CRISPR Mouse - Deletion 3T3-L1 SWELL1