protein-isolation-tissue-mouse-heart

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Mouse lung tissue

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Heart

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Mouse Heart

Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - heart

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Heart

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using PAXgene Tissue RNA/miRNA Kit to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using MagMAX™-96 Total RNA Isolation Kit to perform RNA isolation / purification Tissue - rat heart tissue

Get tips on using BHI - Brain Heart Infusion to perform Bacterial cell culture media Salmonella enterica