QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Use only the Dpn I enzyme provided; do not substitute with an enzyme from another source.

Protocol tips
- Use only the Dpn I enzyme provided; do not substitute with an enzyme from another source.

Publication protocol

Luciferase assay
Cells were co-transfected with pCMV-Renilla along with following plasmids: 1.5BV Id1-luciferase (kind gift from Dr. Benezra) and Oct-4 luciferase (described in16). MEF binding site were mutated using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent technologies). Following primers were synthesized using a software from https://www.genomics.agilent.com/ for site-directed mutagenesis:

mId1mt-F: 5′-agttcatttctctagaaatttgagtcaggcattccgttcagagtaaaagagagcctgctttgaaatc-3′ mId1mt-R: 5′-gatttcaaagcaggctctcttttactctgaacggaatgcctgactcaaatttctagagaaatgaact-3′.

The luminescence of the lysates was measured using Dual-Luciferase Reporter Assay System (Promega) and read by Veritas microplate luminometer (Turner Biosystems). The Firefly luminescence was normalized to Renilla luminescence.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Site Directed Mutagenesis (SDM) Human - Point mutation U-87MG MEF using QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn from Agilent Technologies.

Manufacturer protocol

Download the product protocol from Agilent Technologies for QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Site Directed Mutagenesis (SDM) Human - Point mutation U-87MG MEF using QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn from Agilent Technologies. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.