QuikChange Site-Directed Mutagenesis Kit, 10 Rxn

Site Directed Mutagenesis (SDM) Human - Point mutation Huh7 Reptin

Experiment
Site Directed Mutagenesis (SDM) Human - Point mutation Huh7 Reptin
Product
QuikChange Site-Directed Mutagenesis Kit, 10 Rxn from Agilent Technologies
Manufacturer
Agilent Technologies

Protocol tips

Upstream tips
- XL10-Gold cells are resistant to tetracycline and chloramphenicol. If the mutagenized plasmid contains only the tetR or camR resistance marker, an alternative strain of competent cells must be used.
Protocol tips
- Use only the Dpn I enzyme provided; do not substitute with an enzyme from another source.

Publication protocol

The D299N mutant Flag-Reptin cDNA was a gift from M
Cole (Lebanon, USA). The E300G mutant was constructed with the QuikChange site-directed mutagenesis kit from Agilent Technologies (Massy, France) and verified by sequencing. Mutants and WT Flag-Reptin were inserted in the previously described lentiviral vector pRRLSIN-MNDMCS-WPRE (8). Titers were determined by the transduction of HEK 293T cells through the serial dilution of the lentiviral supernatant and were analyzed for p24 protein expression 5 days later.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Site Directed Mutagenesis (SDM) Human - Point mutation Huh7 Reptin using QuikChange Site-Directed Mutagenesis Kit, 10 Rxn from Agilent Technologies.

Paper title
Induced CYP3A4 expression in confluent Huh7 hepatoma cells as a result of decreased cell proliferation and subsequent pregnane X receptor activation.
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Manufacturer protocol

Download the product protocol from Agilent Technologies for QuikChange Site-Directed Mutagenesis Kit, 10 Rxn below.

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