QuikChange Site-Directed Mutagenesis Kit, 10 Rxn

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- XL10-Gold cells are resistant to tetracycline and chloramphenicol. If the mutagenized plasmid contains only the tetR or camR resistance marker, an alternative strain of competent cells must be used	- Use only the Dpn I enzyme provided; do not substitute with an enzyme from another source.

Upstream tips
- XL10-Gold cells are resistant to tetracycline and chloramphenicol. If the mutagenized plasmid contains only the tetR or camR resistance marker, an alternative strain of competent cells must be used
Protocol tips
- Use only the Dpn I enzyme provided; do not substitute with an enzyme from another source.

Publication protocol

2.5. Site-Directed Mutagenesis of AP1 and NFκB Sites of the AhR Promoter
AhR promoter analysis with Mathinspector software (Genomatix Software, Germany) revealed the presence of 3 AP1 and 3 NFκB putative binding sites. These sites were mutated using the QuickChange site-directed mutagenesis kit (Stratagene, France). Sequences of sense primers used for mutagenesis are listed in Table 2. Presence of the mutations was checked by restriction analysis and verified by DNA sequencing.

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Manufacturer protocol

Download the product protocol from Agilent Technologies for QuikChange Site-Directed Mutagenesis Kit, 10 Rxn below.

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