Q5® Site-Directed Mutagenesis Kit

Site Directed Mutagenesis (SDM) Mouse - C2C12 miR-145 target site

Experiment

Site Directed Mutagenesis (SDM) Mouse - C2C12 miR-145 target site

Product

Q5® Site-Directed Mutagenesis Kit from New England BioLabs

Manufacturer

New England BioLabs

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Only use 1 μl of PCR product in the KLD reaction. Carrying too much PCR product forward can decrease transformation efficiency. - Only use 5 μl of the KLD reaction in the transformation. If more KLD reaction is added, a buffer exchange step, such as PCR purification, should be included prior to transformation.

Protocol tips
- Only use 1 μl of PCR product in the KLD reaction. Carrying too much PCR product forward can decrease transformation efficiency. - Only use 5 μl of the KLD reaction in the transformation. If more KLD reaction is added, a buffer exchange step, such as PCR purification, should be included prior to transformation.

Publication protocol

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Reviews

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Papers

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Manufacturer protocol

Download the product protocol from New England BioLabs for Q5® Site-Directed Mutagenesis Kit below.

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Videos

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