Proteome Profiler™ Human Apoptosis Array Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	T cells were treated with or without CCF52 ganglioside (15μg/ml) for 48hrs. Thereafter, cell lysates were subjected to analysis using the Proteome Profiler human apoptosis antibody array kit from R&D Systems (Minneapolis, MN) according to the manufacturer’s instructions. Arrays were developed with streptavidin-HRP for 30min on a rocking platform shaker. Developed signals were densitized using Image J software, pixel densities were normalized to untreated sample and expressed as mean pixel density.

Protocol tips

T cells were treated with or without CCF52 ganglioside (15μg/ml) for 48hrs. Thereafter, cell lysates were subjected to analysis using the Proteome Profiler human apoptosis antibody array kit from R&D Systems (Minneapolis, MN) according to the manufacturer’s instructions. Arrays were developed with streptavidin-HRP for 30min on a rocking platform shaker. Developed signals were densitized using Image J software, pixel densities were normalized to untreated sample and expressed as mean pixel density.

Publication protocol

T cells were treated with or without CCF52 ganglioside (15μg/ml) for 48hrs. Thereafter, cell lysates were subjected to analysis using the Proteome Profiler human apoptosis antibody array kit from R&D Systems (Minneapolis, MN) according to the manufacturer’s instructions. Arrays were developed with streptavidin-HRP for 30min on a rocking platform shaker. Developed signals were densitized using Image J software, pixel densities were normalized to untreated sample and expressed as mean pixel density.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Apoptosis assay cell type - Array of apoptotic proteins using Proteome Profiler™ Human Apoptosis Array Kit from R&D system, Minneapolis, MN, USA.

Manufacturer protocol

Download the product protocol from R&D system, Minneapolis, MN, USA for Proteome Profiler™ Human Apoptosis Array Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Apoptosis assay cell type - Array of apoptotic proteins using Proteome Profiler™ Human Apoptosis Array Kit from R&D system, Minneapolis, MN, USA. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.