In Situ Cell Death Detection Kit, Fluorescein

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cells were pre- treated with various concentrations of rigosertib for 48 hours and stained with TUNEL reaction mixture		- When Nonspecific labelling is present, reduce concentration of TdT - When high background is present reduce concentration of labelling mix by 50%

Upstream tips
- Cells were pre- treated with various concentrations of rigosertib for 48 hours and stained with TUNEL reaction mixture
Downstream tips
- When Nonspecific labelling is present, reduce concentration of TdT - When high background is present reduce concentration of labelling mix by 50%

Publication protocol

Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was conducted using Cell Death Detection Kit, Fluorescein (Roche Diagnostics, Basel, Switzerland). Cells were treated as described above with various concentrations of rigosertib for 48 hours and stained with TUNEL reaction mixture per the manufacturer's instructions. Staining was quantified using a FACSVANTAGE™ flow cytometer (BD Biosciences, San Jose, CA) or images were captured by fluorescent microscopy.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for In Situ Cell Death Detection Kit, Fluorescein below.

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