Phusion Site-Directed Mutagenesis Kit

Site Directed Mutagenesis (SDM) Human - Deletion MDA-MB-231 sodium channel β1 subunit

Experiment
Site Directed Mutagenesis (SDM) Human - Deletion MDA-MB-231 sodium channel β1 subunit
Product
Phusion Site-Directed Mutagenesis Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Phusion Hot Start II DNA Polymerase tends to work better at elevated denaturation and annealing temperatures due to higher salt concentrations in its buffer. Hence follow manufacturer instruction strictly.

Publication protocol

Deletion of β1 Ig domain
The Ig domain of β1 (amino acids 40–124) in pEGFPN124 was deleted using the Phusion site-directed mutagenesis kit (Thermo Scientific). The β1Δ40–124-GFP construct was sequenced, and then transfected into MDA-MB-231 cells using Fugene (Roche).24

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Phusion Site-Directed Mutagenesis Kit below.

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