QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric

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	Starve cells by incubating 18-24 hours prior to assay in appropriate serumfree medium (DMEM, EMEM, or equivalent

Protocol tips
Starve cells by incubating 18-24 hours prior to assay in appropriate serumfree medium (DMEM, EMEM, or equivalent

Publication protocol

Cell invasion was measured using a QCMTM 24-well Fluorometric Cell Invasion Assay kit (Millipore), which is composed of 85% type I and 15% type III collagen according to the manufacturer’s instruction. Briefly, cells were seeded onto an invasion chamber insert containing an 8-μm pore size polycarbonate membrane coated with a thin layer of polymerized collagen. Invading cells on the bottom of the insert membrane were visualized and counted under the DM 2500 fluorescence microscope.

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Manufacturer protocol

Download the product protocol from Merck Millipore for QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric below.

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