Cultrex® BME Cell Invasion Assay

Protocol tips

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	Add 30% FBS medium as chemoattractant. Incubate at 37C in a CO2 incubator for 6 hours

Protocol tips
Add 30% FBS medium as chemoattractant. Incubate at 37C in a CO2 incubator for 6 hours

Publication protocol

Cultrex 96 Well Cell Migration Assay kit and Cultrex 96 Well BME Cell Invasion Assay kit were purchased from Trevigen, Gaithersburg, MD and were used for cell migration and cell invasion assays, respectively, following the manufacturer’s instructions. 30% FBS medium was used as chemoattractant. Migration was assessed via fluorescence measurement after 6 hours at 485nm excitation and 520 nm emission, using Tecan Infinite microplate reader. For the invasion assay, the chambers were coated with 0.1x BME solution, and invasion was assessed after 24 hours via fluorescence measurement.

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Manufacturer protocol

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