96-Well Cell Invasion Assay, Collagen I

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 5 × 105 cells	Cover the plate and transfer to a cell culture incubator for 24 hours	Read the fluorescence with a fluorescence plate reader at 560 nm.

Upstream tips
Seed 5 × 105 cells
Protocol tips
Cover the plate and transfer to a cell culture incubator for 24 hours
Downstream tips
Read the fluorescence with a fluorescence plate reader at 560 nm.

Publication protocol

Cell invasion was measured using the CytoSelect 96-Well Collagen I Cell Invasion Assay (Cell Biolabs). Cells (5 × 105) were seeded in serum-free media onto polycarbonate membrane inserts (8 μm thick) whose upper surfaces were coated with a uniform layer of dried Bovine Type I Collagen matrix (Cell Biolabs). Inserts were then submerged in media containing 10% fetal bovine serum (FBS), and the cells were cultured for 24 h. Invading cells were stained with cell stain solution, followed by measurement using a Fluoroskan Ascent plate reader (Thermo Scientific) at 560 nm.

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