Culture-Insert 4 Well in µ-Dish 35 mm, high

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 70 μl of 5 × 105 cells/ml	Incubate at 37°C and 5 % CO2 for 24 h

Upstream tips
Seed 70 μl of 5 × 105 cells/ml
Protocol tips
Incubate at 37°C and 5 % CO2 for 24 h

Publication protocol

For the MCF-7 cell migration assay, an IBIDI culture insert (IBIDI GmbH) was placed into a 35- mm culture dish and slightly pressed on top to ensure tight adhesion. An equal number of control and fascin-1-silenced MCF-7 cells (70 μl; 5 × 105 cells/ml) were seeded into the two reservoirs of the same insert and incubated at 37°C in a 5% CO2 humidified incubator. After 24 h, the insert was gently removed, creating a gap of about 500 μm. Cells were then cultured in DMEM medium without FBS and incubated with 100 ng/ml TPA for another 24 h. Cells were then photographed (100×magnification) to monitor cell migration into the wounded area, and the width of the cell-free zone (distance between the edges of the injured monolayer) was calculated.

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Manufacturer protocol

Download the product protocol from Ibidi for Culture-Insert 4 Well in µ-Dish 35 mm, high below.

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