QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), colorimetric

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 1000 cells in 24-well filter-inserts,	Cover plate and incubate for 8 hours at 37°C in a CO2 incubator (4-6% CO2).

Upstream tips
Seed 1000 cells in 24-well filter-inserts,
Protocol tips
Cover plate and incubate for 8 hours at 37°C in a CO2 incubator (4-6% CO2).

Publication protocol

Migration assays were performed with the QCM Colorimetric Cell Migration Assay kit according to the manufacturer's protocol (Millipore, Billerica, MA, USA). MCF-7 cells were transfected with control vector, KLLN-containing plasmid or anti-KLLN siRNA. After 48 h, 1000 cells were seeded in 24-well filter-inserts, and allowed to migrate toward 10% FBS for 8 h. Colorimetric measurements were taken according to the assay instructions

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Manufacturer protocol

Download the product protocol from Merck Millipore for QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), colorimetric below.

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