ORIS™ Cell Migration Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 24 hours to permit cell attachment.

Protocol tips
Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 24 hours to permit cell attachment.

Publication protocol

Chemostatic cell movement was analyzed using an Oris™ cell migration assay kit (Platypus Technologies, Madison, WI) as described previously [48]. For the chemotactic cell migration assay, a Transwell-chamber insert (Corning Costar, Cambridge, MA) with filters precoated on the lower surface with gelatin was placed in a 24-well tissue culture plate containing conditioned medium from NIH3T3 fibroblasts cultured in complete medium. Cells (2 × 104) suspended in serum-free RPMI medium were added to the upper chambers. Following a 24 hour incubation, cells were fixed and stained with crystal violet. After removal of non-migrated cells on the upper surface of the filter, chemotactic cell motility was quantified by counting the cells on the lower surface of the filter.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - DU145 using ORIS™ Cell Migration Assay from Platypus Technologies.

Manufacturer protocol

Download the product protocol from Platypus Technologies for ORIS™ Cell Migration Assay below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell migration / Invasion cell type - DU145 using ORIS™ Cell Migration Assay from Platypus Technologies. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.