Cultrex® BME Cell Invasion Assay

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	Incubate cells for 30 min at 37 °C Lower chamber is with 1% serum Both chambers were assembled and incubated for 4 h

Protocol tips
Incubate cells for 30 min at 37 °C Lower chamber is with 1% serum Both chambers were assembled and incubated for 4 h

Publication protocol

Gelatin pre-coated Transwells (24-wells, 8 μm pore size with polycarbonate membrane; Corning Costar, Cambridge, MA, USA) were used for the Boyden chamber assay.31 Hotchkiss KA, Ashton AW, Mahmood R, et al. Inhibition of endothelial cell function in vitro and angiogenesis in vivo by docetaxel (Taxotere): association with impaired repositioning of the microtubule organizaing center. Mol. Cancer Ther. 2002;1:1191–1200.
[PubMed], [Web of Science ®], , [Google Scholar]
) The upper chamber contained 1  ×  105 cells/mL in M199 medium (1% FBS) and various concentrations of CJME, and cells were incubated for 30 min at 37 °C. The lower chamber contained M199 (1% FBS) and VEGF (20 ng/mL). Both chambers were assembled and incubated for 4 h. After incubation, cells attached to the gelatin surface of the upper chamber were fixed and stained with hematoxylin and eosin (H&E), while non-migrating cells on the upper surface of the membrane were removed with cotton swabs. Migrating cells were counted with an optical microscope (200×).

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