QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), colorimetric

Cell migration / Invasion cell type - FaDu

Experiment
Cell migration / Invasion cell type - FaDu
Product
QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), colorimetric from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
Add 300 µL of prepared cell suspension from step 3 to each insert.

Add 500 µL of serum free media in the presence or absence of chemo-attractant (e.g. 10% fetal bovine serum) to the lower chamber.

Cover plate and incubate for 24 hours at 37°C in a CO2 incubator (4-6% CO2)

Publication protocol

Migration ability was analyzed by wound-healing assay and transwell migration assay. FaDu cells (4 × 105) and Detroit 562 cells (5 × 105) were seeded into 24 well plates. When cells reached a 90–100% confluent monolayer, a scratch was made by a 200 μL pipette tip. Cell debris was removed by phosphate-buffered saline washing after scratching and then cells were incubated in MEM medium with 2% FBS. Images were taken at 0, 12, 18, and 24 h after SPARC treatment. Transwell migration assay was performed in the QCM™ 24-well Cell Migration Assay and Invasion System uncoated 8 m pore size polycarbonate membranes (Millipore) according to the manufacturer’s instructions. Briefly, 8 × 104 FaDu or 1 × 105 Detroit 562 were seeded into 24 wells and inserted into 300 μL serum-free medium while 500 μL medium with 10% FBS was in the lower chamber. After 24 h, the bottom surface of the membrane was fixed in 10% formaldehyde solution, followed by 0.4 g/L crystal violet staining for 2 h. Cells on the upper surface were removed by a cotton swab after membrane washing. The bottom of the membrane was then visualized using the Olympus inverted microscope at 100× magnification. Four random fields of view were counted and the relative fold of migration in each group was compared to the 0 ng/mL SPARC treated group.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - FaDu using QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), colorimetric from Merck Millipore.

Paper title
Secreted Protein Acidic and Rich in Cysteine (SPARC) Enhances Cell Proliferation, Migration, and Epithelial Mesenchymal Transition, and SPARC Expression is Associated with Tumor Grade in Head and Neck Cancer
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Manufacturer protocol

Download the product protocol from Merck Millipore for QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), colorimetric below.

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