24-Well Cell Invasion Assays, Basement Membrane

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate for 24-48 hours at 37ºC in 5% CO2 atmosphere.	Measure the OD 560nm in a plate reader

Protocol tips
Incubate for 24-48 hours at 37ºC in 5% CO2 atmosphere.
Downstream tips
Measure the OD 560nm in a plate reader

Publication protocol

The wound healing assay was performed with a Cytoselect 24-Well Cell Invasion Assay Kit (Cell Biolabs, San Diego, CA, USA) according to the manufacturer's protocol. Transfected cells were added to either side of the open end at the top of the insert. When the cells had formed a monolayer, the insert was removed to generate a consistent 0.9-mm gap wound in the middle. To determine migration distance, the size of the wound was measured at each time point. At multiple time points, cells were fixed and stained with methylene blue and photographed.

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